Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Dec 12;456:140930. doi: 10.1016/j.cej.2022.140930

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 Elsevier B.V. All rights reserved.

Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

PMC Copyright notice

Fig. 2 — Characterization and mRNA delivery efficiency of F-PEI. (a) Agarose gel electrophoresis of F-PEI/mRNA at different w/w ratios. (b) Dynamic light scattering analysis of F-PEI/mRNA. (c) Transmission electron microscopy imaging shown F₁₃-PEI_1.8k-1/mRNA and F₁₃-PEI_1.8k-2/mRNA. Scale bar = 200 nm. (d) Zeta potentials of the F₁₃-PEI_1.8k-1, F₁₃-PEI_1.8k-2, mRNA, F₁₃-PEI_1.8k-1/mRNA and F₁₃-PEI_1.8k-2/mRNA. (e&f) Representative flow cytometry plots (e) and the mean fluorescence intensity (MFI) of Cy5 (f) in BMDCs incubated with F-PEI/Cy5-mRNA^Luc for 6 h. (g&h) F-PEI/mRNA^Luc (10 μg mRNA^Luc per mouse) was intradermally injected at the tail base of mice to evaluate the in vivo mRNA delivery efficiency, and the bioluminescence images (g) and statistical data (h) were recorded at 6 h and 24 h post injection. d,f, The data show mean ± standard deviation (n = 3). h, The data show mean ± standard error of mean (n = 3 mice per group). f,h, Statistical significance between the indicated groups was determined using two-sided unpaired t-tests. **P < 0.01, ***P < 0.001.