FIG. 1.

Expression of serotype O1 partial antigens by an encapsulated parent Klebsiella strain and its decapsulated mutant. LPS was prepared from strain 591 (lanes 1, 3, 5, and 7) and its decapsulated mutant (lanes 2, 4, 6, and 8). (A) Silver stain; (B) Western blot reacted with MAb Ru-O1; (C) Western blot reacted with MAb IV/4-5; (D) Western blot reacted with MAb V/9-5. Both the parent strain and the decapsulated mutant expressed all three known partial antigens of the O1 serogroup. Similar reactions were obtained with the other strains used in this study.