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. Author manuscript; available in PMC: 2023 Nov 9.
Published in final edited form as: Cell Host Microbe. 2022 Nov 1;30(11):1589–1601.e5. doi: 10.1016/j.chom.2022.10.004

Figure 2. lip2 induces an elevated IL-17 response in kidneys.

Figure 2.

(A) lip2 provokes an exaggerated Il17a response in infected kidneys. Groups of female BALB/c mice were infected with 1×105 CFU of WT or lip2, followed by euthanasia of three animals per group at the indicated time points. Il1b, Il6, Il10, Ifng, Tnf, Il17a mRNA expression was assessed by RT-qPCR of RNA recovered from kidney homogenates (expression relative to Gadph). Statistical significance of differences between WT-infected kidneys and lip2-infected kidneys was determined by an unpaired two-tailed t-test. * p<0.05; ** p<0.01; *** p<0.001; **** p<0.0001. (Note that points without visible error bars displayed SEMs smaller than the circle.)

(B) lip2 induces a strong renal IL-17A response during systemic infection. Groups of BALB/c mice were infected with 1×105 CFU WT or lip2 followed by euthanasia of three animals per group at the indicated time points. Il17a mRNA was measured by RT-qPCR in the right kidney homogenates and normalized to Gadph (left panel). IL-17A protein production was evaluated by ELISA in the left kidney homogenates (right panel). Statistical significance between WT and lip2 was determined by an unpaired two-tailed t-test. ns: p=0.1797; ** p<0.01; *** p<0.001; **** p<0.0001.