KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Brilliant Violet 785 anti-mouse CD11b (M1/70) | Biolegend | 101243 |
| PE-Cy7 anti-mouse CD11c (N418) | Biolegend | 117317 |
| Pacific Blue anti-mouse CD45.2 (104) | Biolegend | 109819 |
| Brilliant Violet 785 anti-mouse CD90.2 (30-H12) | Biolegend | 105331 |
| Brilliant Violet 605 anti-mouse CD45R/B220 (RA3-6B2) | Biolegend | 103243 |
| PE anti-mouse IL-17A (TC11-18H10.1) | Biolegend | 506903 |
| PerCP/Cyanine5.5 anti-mouse TCR gamma delta (GL3) | Biolegend | 118117 |
| PE anti-mouse MHCII (I-A/I-E) (M5/114.15.2) | Biolegend | 50-5321-U025 |
| FITC anti-mouse CD4 (GK1.5) | Biolegend | 100405 |
| Brilliant Violet 785 anti-mouse CD11b (M1/70) | Biolegend | 101243 |
| PE-Cy7 anti-mouse CD11c (N418) | Biolegend | 117317 |
| Pacific Blue anti-mouse CD45.2 (104) | Biolegend | 109819 |
| anti-FLAG | Thermo Fisher Scientific | MA1-142-A488 |
| Chemicals, peptides, and recombinant proteins | ||
| Acid phenol chloroform | Ambion | AM9722 |
| Phenol chloroform isoamyl alcohol | Ambion | AM9732 |
| Recombinant mouse GM-CSF | Prepotech | Cat# 315-03 |
| IL-4 | Gibco | Cat# PMC0045 |
| Pam3CSK4 | InvivoGen | Cat# tlrl-pms |
| Poly(I:C) | InvivoGen | Cat# tlrl-pic |
| LPS from E.coli | Sigma | Cat# L3024 |
| imiquimod | Calbiochem | Cat# CAS 99001-02-6 |
| Palmitic acid | Sigma | Cat# P0500 |
| Stearic acid | Sigma | Cat# S4751 |
| Linoleic acid | Sigma | Cat #L1376 |
| RPMI media | UCSF Media Production | Cat# CCFAE001 |
| FBS | UCSF Media Production | Cat# CCFAQ009 |
| Bovine serum Albumin | Sigma | Cat# A6003 |
| Percoll | Cytivia | Cat# 17089101 |
| PMA | Sigma | Cat# P1585 |
| ionomycin | Sigma | Cat# I9657 |
| GolgiStop | Fisher Scientific | Cat# BDB555029 |
| BD Cytofix buffer | Fisher Scientific | Cat# BDB554655 |
| Perm/Wash reagent | BD Biosciences | Cat# BDB554723 |
| Critical commercial assays | ||
| Collagenase I | Thermo Fisher Scientific | Cat# 17100017 |
| DNAse I | Thermo Fisher Scientific | Cat# EN0521 |
| RNeasy Plus mini kit | Qiagen | Cat# 74134 |
| DuoSet ELISA kit IL-23 | R&D Systems | Cat# DY1887 |
| DuoSet ELISA kit IL-17 | R&D Systems | Cat# DY421 |
| DuoSet ELISA kit IL-1 | R&D Systems | Cat# DY401 |
| DuoSet ELISA kit IL-6 | R&D Systems | Cat# DY406 |
| Lipase Assay Kit | Sigma | Cat# MAK046 |
| pNp-palmitate | Sigma | Cat# N2752 |
| Trizol reagent | ThermoFisher | Cat# 15596026 |
| SYBR Green Master Mix | Biorad | Cat# 1725275 |
| Superscript III | Invitrogen | Cat# 18080 |
| Deposited data | ||
| Experimental models: Cell lines | ||
| Bone marrow-derived dendritic cells differentiated in the presence of GM-CSF and IL-4 | This paper | N/A |
| Experimental models: Organisms/strains | ||
| Mouse: BALB/c | Charles River Laboratories | Stock No: 028 |
| Mouse: C57BL/6J | Jackson Laboratories | Stock no. 000664 |
| Mouse: il17af−/− | Jackson Laboratories | Stock no. 034140 |
| SN2019 | this study | WT control strain for lip2DOX-OFF |
| SN2464 | Roemer et al. (2003) | lip2 DOX-OFF |
| SN250 | Noble et al. (2010) | leu2Δ::C.d.LEU2/leu2Δ::C.m.HIS1, his1Δ/his1Δ, arg4Δ/arg4Δ, ura3Δ/URA3, iro1/IRO1 |
| SN2461 | Noble et al. (2010) | lip2Δ::C.d.LEU2/lip2Δ::C.m.HIS1, his1Δ/his1Δ, arg4Δ/arg4Δ, ura3Δ/URA3, iro1/IRO1 |
| SN2242 | This study | LIP2/lip2Δ, leu2Δ::C.d.LEU2/leu2Δ::C.m.HIS1, his1Δ/his1Δ, arg4Δ::C.d.ARG4/arg4Δ, ura3Δ/URA3, iro1/IRO1 |
| SN2243 | This study | LIP2S196A/lip2Δ, leu2Δ::C.d.LEU2/leu2Δ::C.m.HIS1, his1Δ/his1Δ, arg4Δ::C.d.ARG4/arg4Δ, ura3Δ/URA3, iro1/IRO1 |
| SN2246 | This study | LIP2D240A/lip2Δ, leu2Δ::C.d.LEU2/leu2Δ::C.m.HIS1, his1Δ/his1Δ, arg4Δ::C.d.ARG4/arg4Δ, ura3Δ/URA3, iro1/IRO1 |
| SN2250 | This study | LIP2H344A/lip2Δ, leu2Δ::C.d.LEU2/leu2Δ::C.m.HIS1, his1Δ/his1Δ, arg4Δ::C.d.ARG4/arg4Δ, ura3Δ/URA3, iro1/IRO1 |
| SN2253 | This study | LIP2S196A,D240A,H344A/lip2Δ, leu2Δ::C.d.LEU2/leu2Δ::C.m.HIS1, his1Δ/his1Δ, arg4Δ::C.d.ARG4/arg4Δ, ura3Δ/URA3, iro1/IRO1 |
| SN119 | Noble and Johnson (2005) | efg1Δ::C.d.HIS1/efg1Δ::C.m.LEU2, leu2Δ/leu2Δ, his1Δ/his1Δ, ura3Δ/URA3, iro1/IRO1 |
| SN2462 | Noble et al. (2010) | ece1Δ::C.d.LEU2/ece1Δ::C.m.HIS, leu2Δ/leu2Δ, his1Δ/his1Δ, arg4Δ/arg4Δ, ura3Δ/URA3, iro1/IRO1 |
| SN1664 | Noble et al. (2010) | sap6Δ::C.d.LEU2/sap6Δ::C.m.HIS, leu2Δ/leu2Δ, his1Δ/his1Δ, arg4Δ/arg4Δ, ura3Δ/URA3, iro1/IRO1 |
| SN2463 | Noble et al. (2010) | lip10Δ::C.d.LEU2/lip10Δ::C.m.HIS, leu2Δ/leu2Δ, his1Δ/his1Δ, arg4Δ/arg4Δ, ura3Δ/URA3, iro1/IRO1 |
| Oligonucleotides | ||
| Oligonucleotides are described in Table S1 | N/A | N/A |
| Software and algorithms | ||
| Other | ||