Table 3. The results show the effects on protein stability and TM-align score of 19 high-risk missense variations of the CYP1B1.

rs ID	Variant	I-Mutant 2.0		MUpro		TM Align		gnomAD	Population
		DDG	Stability	DDG	Stability	RMSD	TM score	Allele frequency
rs201824781	P52L	0.59	Decrease	0.470	Increase	1.47	0.971	0.000299/42	Ashkenazi Jewish, European, Latino, African, others
rs28936700	G61E	−0.2	Increase	−0.133	Decrease	1.2	0.984	0.000171/24	European, South Asian, Latino, others
rs112014184	G90R	−1.56	Decrease	−0.606	Decrease	0.98	0.989	Not available	Not available
rs73625096	P118L	0.68	Increase	−0.123	Decrease	1.53	0.978	0.000005/1	Latino
rs72481807	E173K	−0.24	Decrease	−0.998	Decrease	1.3	0.980	0.000007/1	Others
rs72480439	D291G	−1.26	Decrease	−2.096	Decrease	1.34	0.980	0.000008/2	East Asian
rs200182794	Y349D	−0.03	Increase	−1.239	Decrease	1.26	0.985	0.000008/2	African, European (non-Finnish)
rs55771538	G365R	−1.02	Decrease	−0.502	Decrease	1.38	0.979	0.000004/1	European (non-Finnish)
rs55771538	G365W	−0.66	Decrease	−0.606	Decrease	1.49	0.978	0.000004/1	European (non-Finnish)
rs72480442	R368C	−0.8	Decrease	−0.59	Decrease	1.22	0.979	0.000207/29	Latino. European (non-Finnish), others
rs79204362	R368H	−1.53	Decrease	−0.708	Decrease	1.34	0.978	0.001248/175	South Asian, East Asian, Ashkenazi Jewish, Latino, European, African
rs104893622	D374N	−0.74	Decrease	−0.62	Decrease	1.27	0.979	Not available	Not available
rs104893629	N423Y	0.02	Decrease	−0.750	Decrease	1.4	0.979	0.000007/1	European (non-Finnish)
rs201181935	D430E	−0.46	Increase	−0.919	Decrease	1.31	0.978	0.000007/1	East Asian
rs72481808	P442A	−1.07	Decrease	−0.72	Decrease	0.68	0.993	Not available	Not available
rs72549376	R444Q	−1.39	Decrease	−1.35	Decrease	1.41	0.977	0.000007/1	European (non-Finnish)
rs148233007	F445L	−2.41	Decrease	−0.856	Decrease	1.37	0.982	0.000007/1	South Asian, European (non-Finnish)
rs28936701	R469W	−0.75	Decrease	−0.577	Decrease	1.38	0.977	0.000036/5	South Asian, East Asian, European (non-Finnish), Latino
rs104894979	C470Y	0.33	Decrease	−1.111	Decrease	1.42	0.977	Not available	Not available