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. 2022 Dec 2;10:e14472. doi: 10.7717/peerj.14472

Figure 2. CRISPR/Cas9 mediated generation of Ctsb-/- mice.

Figure 2

(A) Schematic representation of targeting strategy using CRISPR/Cas9 system. Two sgRNA target sites were indicated, and the deletion between the sgRNA target sites was identified by the forward (F) and reverse (R1, R2) primers. (B) DNA sequencing chromatograms showing a 9819bp deletion in Ctsb-/- mice. (C) Agarose gel electrophoresis analysis showing DNA bands for the different genotypes. (D) Western blot showing no band at the expected size of the CTSB protein in Ctsb-/- mice. (E) Fertility test showing the number of pups per litter from four WT and four Ctsb-/- males. Each black dot indicates the number of mice born in each litter. * P < 0.05 vs WT.