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. 2022 Dec 2;10:e14472. doi: 10.7717/peerj.14472

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©2022 Wen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

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(A) The morphology of the testis, epididymis, and seminal vesicle in WT and Ctsb^-/- mice. (B) The weights of the testis, epididymis, and seminal vesicle did not significantly differ between WT and Ctsb^-/- mice. (C) H & E-stained sections of the testes, caput, and cauda epididymides from WT and Ctsb^-/- mice. (D) Immunofluorescence staining of γH2AX-positive spermatocytes from WT and Ctsb^-/- mice. (E) PNA-stained acrosomes in the developmental spermatid of WT and Ctsb^-/- mice. (F) Immunohistochemistry staining of SOX9-positive Sertoli cells from WT and Ctsb^-/- mice. (G) Sertoli cell counts from WT and Ctsb^-/- mice. NS, non-significant. n ≥ 4 in each group.