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. 2022 Dec 2;10:e14472. doi: 10.7717/peerj.14472

Figure 5. Autophagic and apoptotic activity detected in Ctsb-/- male mice.

Figure 5

(A, B) Western blotting used to detect the protein expression levels of ATG5 and LC3II/LC3I in the testis of WT and Ctsb-/- mice. (C, D) Protein expression levels of ATG5 and LC3II/LC3I in the epididymis of WT and Ctsb-/- mice. (E) Immunohistochemistry staining of LC3 and ATG5 in the testis and caput epididymis from WT and Ctsb-/- mice. Scale bar = 50 µm. (F) Autophagic and apoptotic processes of epididymis cells examined by TEM in WT and Ctsb-/- mice, autophagosomes were indicated. Scale bar = 5 µm. (G) TUNEL assay was used to detect the apoptotic activity in the testis and epididymis of WT and Ctsb-/- mice. Scale bar = 100 µm. (H) Western blot used to detect cleaved-Caspase3 in the testis of WT and Ctsb-/- mice. (I) Western blot used to detect cleaved-Caspase3 in the epididymis of WT and Ctsb-/- mice. * P < 0.05 vs WT, ** P < 0.01 vs WT, NS, non-significant, n ≥ 4 in each group.