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. Author manuscript; available in PMC: 2022 Dec 12.
Published in final edited form as: Cell Rep. 2022 Nov 1;41(5):111576. doi: 10.1016/j.celrep.2022.111576

Figure 3. NUP93 acts as a direct transcriptional activator in the nuclear periphery.

Figure 3.

(A) A diagram of the CRISPR-dCas9-based NUP93 tethering strategy in cells with endogenous NUP93-mAC depleted.

(B) qRT-PCR analysis of the target gene and eRNA expression upon CRISPR-dCas9 tethering of NUP93 to the KITLG promoter or enhancer (with endogenous NUP93 depleted).

(C) qRT-PCR analysis of the target MYC gene and eRNA expression upon CRISPR tethering of NUP93 (with endogenous NUP93 depleted).

(B and C) The bars represent mean ± SD (n = 3 technical replicates). Each plot is a representative example from 3 biological replicates. Student’s t test; *p < 0.05, ***p < 0.001; ns, not significant.

(D) Representative RNA FISH images of CCAT1 in HCT116 cells. White arrowheads point to the positions of RNA FISH signals. Scale bar, 5 μm.

(E) A diagram of subnuclear areas defines the nuclear periphery versus nuclear center. Right panel: quantified percentage of CCAT1 FISH signals in each subnuclear area (n > 200 FISH foci counted).