Table 1. Bacterial strains and plasmids used in this study.
| Strain/plasmid | Description | Source |
|---|---|---|
| Streptococcus pyogenes | ||
| MGAS8232 | M18 serotype isolated from a patient with acute rheumatic fever (GenBank accession: NC_003485.1) | [33] |
| MGAS8232 ΔhasA | hasA deletion mutant derived from MGAS8232 | This study |
| MGAS8232 ΔhasA + hasA | MGAS8232 ΔhasA containing complementation plasmid pDCerm expressing the hasA gene | This study |
| Escherichia coli | ||
| XL1-Blue | General cloning strain | Stratagene |
| Plasmids | ||
| pG+host5 | Temperature-sensitive Gram-positive/E. coli shuttle vector; Ermr | [32] |
| pG+host5::ΔhasA | pG+host5 with hasA flanking regions inserted | This study |
| pDCerm | Ermr derivative of streptococcus-E. coli shuttle vector pDC123 (erm of Tn916ΔE) replacing cat | [35] |
| pDCerm::hasA | pDCerm expressing hasA gene for plasmid-based complementation | This study |
Ermr—erythromycin resistance
cat—chloramphenicol acetyltransferase gene