‘No bootstrap’ corresponds to paraphyetic clustering with bmin = 0; otherwise this threshold defaults to 95%. Optimal dmax is the pairwise distance threshold selected by minimizing ΔAIC, in units of expected number of nucleotide substitutions. ‘Number of clusters’ only counts clusters with two or more background sequences, i.e., this number does not include singletons. Total growth is the number of incident (new) sequences connected to clusters of background sequences. Growing clusters is the number of clusters to which incident sequences attach.