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. Author manuscript; available in PMC: 2023 Jun 2.
Published in final edited form as: Nat Metab. 2022 Jun 2;4(5):524–533. doi: 10.1038/s42255-022-00565-1

Extended Data Fig. 1 |. Mesaconate is endogenously synthesized from itaconate and cellularly permeable in mouse macrophages.

Extended Data Fig. 1 |

a. Intracellular itaconate and mesaconate of BMDMs from wildtype (WT) or IRG1-deficient (KO) mice stimulated with or without LPS for 24 h. b. Intracellular itaconate and mesaconate of BMDMs incubated with exogenous itaconate or mesaconate for the indicated time points. c. Intracellular itaconate and mesaconate of RAW264.7 cells transfected with indicated siRNA, followed with LPS stimulation for 24 h. d. Silencing efficiency of indicated genes from experiments shown in (c). Data are presented as mean ± SEM: a,b. calculated from n = 3 mice from a representative experiment of 2 independent experiments; c,d. n = 6 biological replicates pooled from 2 independent experiments. P values were calculated by one-way ANOVA with Dunnet post-test and overlayed on respective comparisons.