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. Author manuscript; available in PMC: 2023 Jun 2.
Published in final edited form as: Nat Metab. 2022 Jun 2;4(5):524–533. doi: 10.1038/s42255-022-00565-1

Extended Data Fig. 4 |. itaconate derivatives impairs iL-1β secretion in human macrophages.

Extended Data Fig. 4 |

a,b. IL-1β in supernatants of human monocyte-derived macrophages treated with mesaconate, itaconate, DMI or 4-OI of indicated concentrations, LPS stimulation as well as NLRP3 or NLRC4 inflammasome activation by nigericin or a mixture of BsaK and protective antigen, respectively, following the pre- (a) or post-treatment protocol (b) as shown at the top. c. IL-1β in supernatants of human whole blood pre-treated with mesaconate, itaconate, DMI or 4-OI of indicated concentrations for 4 h prior to LPS stimulation overnight. Data are shown as mean ± SEM calculated from n = 5 donors. P values were calculated by one-way ANOVA with Dunnet post-test and overlayed on respective comparisons.