Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | TGF-β (Rabbit polyclonal) | Cell Signaling Technology | Cat# 3711 s | mfIHC(1：800) WB(1:200) IF (1:100) |
| Antibody | TGFBR1 (Rabbit polyclonal) | Abcam | Cat# ab31013 | IF (1:1000) mfIHC(1:2000) WB(1:500) IHC(1:400) |
| Antibody | TGFBR2 (Mouse monoclonal) | Abcam | Cat# ab78419 | WB(1:500) |
| Antibody | SMAD1/5/9 (Rabbit polyclonal) | Abcam | Cat# ab66737 | mfIHC(1:1000) WB(1:400) |
| Antibody | pSMAD1/5/9 (Ser 463/Ser465) (Rabbit polyclonal) | Santa Cruz Biotechnology | Cat# sc-12353 | mfIHC(1：500) |
| Antibody | SMAD4 (Mouse monoclonal) | Cell Signaling Technology | Cat# 46535 | mfIHC(1：1000) |
| Antibody | SMAD2/3 (Rabbit polyclonal) | Santa Cruz Biotechnology | Cat# sc-8332 | mfIHC(1：400) |
| Antibody | pSMAD2/3 (Ser 423/425) (Rabbit polyclonal) | Santa Cruz Biotechnology | Cat# sc-11769 | mfIHC(1：1000) |
| Antibody | SMAD6 (Rabbit polyclonal) | Santa Cruz Biotechnology | Cat# sc-25321 | WB(1:200) |
| Antibody | Cytokeratin (CK) (Mouse monoclonal) | Santa Cruz Biotechnology | Cat# sc-57004 | mfIHC(1：200) WB(1:500) |
| Antibody | STAT1 (Rabbit polyclonal) | Cell Signaling Technology | Cat# 14994 | WB(1:500) |
| Antibody | pSTAT1(Tyr701) (Rabbit polyclonal) | Cell Signaling Technology | Cat# 9167 | WB(1:500) |
| Antibody | ERK (Rabbit polyclonal) | Cell Signaling Technology | Cat# 4695 S | WB(1:200) |
| Antibody | pERK (Rabbit polyclonal) | Cell Signaling Technology | Cat# 4376 S | WB(1:200) |
| Antibody | Vimentin (Rabbit monoclonal) | Abcam | Cat# ab92547 | WB(1:100) |
| Antibody | E-cadherin (Rabbit polyclonal) | Abcam | Cat# ab15148 | WB(1:100) |
| Antibody | N-cadherin (Rabbit polyclonal) | Abcam | Cat# ab12221 | WB(1:100) |
| Antibody | GAPDH (Mouse monoclonal) | Beyotime | Cat# AG019-1 | WB(1:1000) |
| Antibody | β-actin (Mouse monoclonal) | Beyotime | Cat# AF0003 | WB(1:1000) |
| Antibody | CD4 (Rabbit monoclonal) | Abcam | Cat# ab133616 | IHC(1:200) mfIHC(1:1000) |
| Antibody | CD8 (Mouse monoclonal) | Santa Cruz Biotechnology | Cat# sc-53212 | IHC(1:500) mfIHC(1:200) |
| Antibody | FOXP3 (Rabbit polyclonal) | Cell Signaling Technology | Cat# 98377 | IHC(1:200) mfIHC(1:800) |
| Antibody | OAS1 (Rabbit polyclonal) | Cell Signaling Technology | Cat# 14498 | IHC(1:100) |
| Antibody | IFI27 (Rabbit polyclonal) | Abclonal | Cat# A14174 | WB(1:100) |
| Antibody | RPS3 (Rabbit monoclonal) | Abcam | Cat# ab128995 | WB(1:200) |
| Antibody | IFNAR1 (Rabbit polyclonal) | Abcam | Cat# ab45172 | WB(1:100) |
| Antibody | PDL1 (Rabbit monoclonal) | Abcam | Cat# ab205921 | WB (1:100) |
| Antibody | Ki67 (Rabbit polyclonal) | ZSGB-Bio | Cat# ZM-0166 | IHC (1:1000) |
| Antibody | Human CD3 (OKT3) FG (Mouse monoclonal) | eBioscience | Cat# 16-0037-85 | (1:100) |
| Antibody | Human CD28 FG 16-0289-85 (Mouse monoclonal) | eBioscience | Cat# 16-0289-85 | |
| Antibody | Human FITC CD3 (Mouse monoclonal) | BD Pharmingen | Cat# 555332 | FCM(1:100) |
| Antibody | Human APC CD4 (Mouse monoclonal) | BD Pharmingen | Cat# 555349 | FCM(1:100) |
| Antibody | Human PE CD8 (Mouse monoclonal) | BD Pharmingen | Cat# 555637 | FCM(1:100) |
| Antibody | Ultra-LEAF purified anti-mouse CD3 Antibody (Rabbit monoclonal) | BioLegend | Cat# 100239 | FCM (1:100) |
| Antibody | Ultra-LEAF purified anti-mouse CD28 Antibody (Mouse monoclonal) | BioLegend | Cat# 122021 | (1:100) |
| Antibody | Mouse FITC CD3 (Rabbit monoclonal) | BioLegend | Cat# 100204 | FCM(1:100) |
| Antibody | Mouse PE CD8a (Rabbit monoclonal) | BioLegend | Cat# 100708 | FCM(1:100) |
| Antibody | Mouse APC CD44 (Rabbit monoclonal) | BioLegend | Cat# 103012 | FCM(1:100) |
| Antibody | Mouse BV421 CD62L (Rabbit monoclonal) | BioLegend | Cat# 104435 | FCM(1:100) |
| Antibody | Mouse APC CD279 (PD-1) (Rabbit monoclonal) | BioLegend | Cat# 135209 | FCM(1:100) |
| Antibody | Mouse PE/Cy7 CD107a(LAMP-1) (Rabbit monoclonal) | BioLegend | Cat# 121619 | FCM(1:100) |
| Antibody | Mouse PE/Cy7 CD197 (CCR7) (Rabbit monoclonal) | BioLegend | Cat# 353211 | FCM(1:100) |
| Antibody | InVivoPlus Anti-mouse PD-1 (Mouse monoclonal) | BiXcell | Cat# BP0273 | (1:100) |
| Antibody | InVivoPlus Anti-mouse/human TGF-β (Mouse monoclonal) | BiXcell | Cat# BP0057 | (1:100) |
| Antibody | Human IFN-alpha/beta R1 PE-conjugated antibody (Mouse monoclonal) | R&D | Cat# FAB245P | FCM(1:100) |
| Biological samples (Homo sapiens) | Human colon cancer tissue microarray | OUTDO | HCol-AS-180Su-14 | |
| Biological samples (H. sapiens) | Human rectal cancer tissue microarray | OUTDO | HRec-Ade180Sur-05 | |
| Biological samples (H. sapiens) | Human colorectal cancer tissue cDNA | OUTDO | HcolA30CS01 | |
| Cell lines (H. sapiens) | HCT116 | Dr. L. Yang (Beijing Chaoyang Hospital) | ||
| Cell lines (H. sapiens) | SW480 | Dr. L. Yang (Beijing Chaoyang Hospital) | ||
| Cell lines (H. sapiens) | RKO | Dr. L. Yang (Beijing Chaoyang Hospital) | ||
| Cell lines (H. sapiens) | HEK293T | Dr. L. Yang (Beijing Chaoyang Hospital) | ||
| cell lines (Mus musculus) | MC38 | PUMC | ||
| Cell lines (M. musculus) | CT26W | PUMC | ||
| Commercial assay or kit | QuantiNova SYBR Green PCR kit | Qiagen | Cat# 208054 | |
| Commercial assay or kit | QuantiTect Reverse Transcription kit | Qiagen | Cat# 205314 | |
| Commercial assay or kit | Poerce BCA Protein Assay Kit | Thermo | Cat# RB230514 | |
| Chemical compound, drug | Lipofectamine 3000 | Invitrogen | Cat# L3000015 | |
| Recombinant proteins | Human Interleukin-2 (hIL-2) | Cell Signaling Technology | Cat# 8907SC | |
| Commercial assay or kit | PerkinElmer Opal 7-color fIHC Kit | PerkinElmer | Cat# NEL797001KT | |
| Commercial assay or kit | Ficoll reagent | Solarbio | Cat# P8900 | |
| Commercial assay or kit | Tumor Dissociation Kit, mouse | Miltenyi Biotec | Cat# 130-091-376 | |
| Commercial assay or kit | CD8a+ T Cell Isolation Kit, mouse | Miltenyi Biotec | Cat# 130-104-075 | |
| Commercial assay or kit | Anti-Human CD8 Magnetic Particles-DM | BD Pharmingen | Cat# 557766 | |
| Commercial assay or kit | HiScribeTM T7 Quick High Yeild RNA Synthesis Kit | NEB | Cat# E2040S | |
| Commercial assay or kit | Monarch RNA Cleanup Kit | NEB | Cat# T2030S | |
| Commercial assay or kit | IP Lysis Buffer | Thermo Fisher | Cat# 87787 | |
| Commercial assay or kit | Pierce RNA 3’ Desthiobiotinylation kit | Thermo Fisher | Cat# 20163 | |
| Commercial assay or kit | PIERCE MAGNETIC RNA PULL-DOWN KIT | Thermo Fisher | Cat# 20164 | |
| Chemical compound, drug | CX-5461 | MCE | Cat# HY-13323 | |
| Recombinant proteins | Human Interferon-α1 | Cell Signaling Technology | Cat# 8927SC | |
| Commercial assay or kit | DIG Northern Starter | Roche | Cat# 12039672910 | |
| Commercial assay or kit | VPS9D1-AS1 FISH Probes | Biosearch | N/A | |
| Commercial assay or kit | Magna RIP RNA-Binding Protein Immunoprecipitation Kit |
Millipore | Cat# 17–700 | |
| Commercial assay or kit | Positively Charged Biodyne Nylon membrane | Invitrogen | Cat# 10100 | |
| Commercial assay or kit | ChIP-IT Express Enzymatic Magnetic Chromatin Immunoprecipitation kit | Active Motif | Cat# 53009 | |
| Commercial assay or kit | Stellaris RNA FISH Wash Buffer A | BIOSEARCH | Cat# SMF-WA1-60 | |
| Commercial assay or kit | Stellaris RNA FISH Wash Buffer B | BIOSEARCH | Cat# SMF-WA1-20 | |
| Commercial assay or kit | Stellaris RNA FISH Hybridization buffers | BIOSEARCH | Cat# SMF-HB1-10 | |
| Commercial assay or kit | Stellaris FISH Probes with Quasar 570 Dye | BIOSEARCH | Cat# SMF-1063–5 | |
| Commercial assay or kit | FITC labeled Goat anti-rabbit secondly antibody | Byotime | Cat# 0652 | |
| Commercial assay or kit | FITC labeled Goat anti-mouse secondly antibody | Byotime | Cat# A0568 | |
| Commercial assay or kit | Antifade mounting medium with DAPI | Solarbio | Cat# S2110 |