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. Author manuscript; available in PMC: 2022 Dec 12.
Published in final edited form as: J Invest Dermatol. 2013 Jan 23;133(8):2041–2049. doi: 10.1038/jid.2013.32

Figure 3. Overexpression of PAX3 or Stat3 inhibits the response of melanoma cells to vemurafenib.

Figure 3

A375 and UACC62 parental cells with pcDNA-3.1-Stat3-CA plasmids or PECE-PAX3 plasmids introduction were stimulated with different doses of vemurafenib as indicated. Total protein was collected 6 hr after stimulation. Protein expressions of Stat3 and PAX3 were analyzed by western blot along with tubulin, which served as a loading control. (A) A375 cells and (B) UACC62 cells. (C) Melanoma cell growth was assessed by MTT assays. Relative growth (RG) was calculated as the ratio of treated to untreated cells at each dose for each replicate.