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Analysis of the expression and secretion of SigA in E. coli carrying the sigA gene. Concentrated culture supernatants were subjected to SDS-PAGE, and the gel was stained with Coomassie blue. Lanes: A, molecular mass markers (indicated in kilodaltons at the left); B, supernatant of DH5α/pPBA1100 (vector only); C, supernatant of DH5α/pSBA479 (sigA). The arrowhead indicates the 103-kDa secreted protein encoded by sigA.
