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Protease activity of SigA. Proteolytic activity of concentrated supernatants derived from E. coli and S. flexneri 2a was measured as described in Materials and Methods. Columns: A, E. coli DH5α/pSBA479 (sigA); B, DH5α/pPBA1100 (vector only); C, S. flexneri 2a YSH6000T (parent); D, SBA1361 (sigA); E, SBA1351 (sigA::kan). Error bars indicate standard errors in assays performed at least in duplicate.
