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. 2022 Dec 12;13:7692. doi: 10.1038/s41467-022-35406-y

Fig. 5. Generation of conditional ST-Cal-Light knock-in mice.

Fig. 5

a Schematic illustration of virus injection and fiber optic implantation. b Plasmid design for generating conditional ST-Cal-Light KI mouse. c Representative images of tdTomato (transfection marker) and EGFP (reporter) and G/R ratio distributions with or without introducing Cre recombinase (Cal-Het w/o Cre: 0.137 ± 0.005 from n = 292 independent cells; Cal-Het w/ Cre: 0.671 ± 0.022 from n = 258 independent cells). Scale bar, 100 μm. The border lines of the box indicate the 25th and 75th percentile, respectively, the horizontal line in the box shows the median and the whiskers mean the minimum and maximum values. Asterisks indicate ***P < 0.001. d Injected viruses (top) and images of EGFP and anti-myc staining. Myc epitope is expressed in a cre-dependent manner and localized at the somatic membranes. EGFP signals indicate active neurons. Scale bars, 50 μm. e Cartoon of the breeding scheme. f A mixture of viruses was injected into the primary motor cortex of Cal-Hom:EMX-Cre mouse (top). Blue light-dependent gene expression in neocortical excitatory neurons under Emx1 promoter (bottom). Scale bars, 50 μm. g Excitatory neuron labeling was confirmed by CaMKII antibody staining. Scale bar, 50 μm. h Virus injection scheme in Cal-Hom:PV-Cre mice. i Schematic flow of generating either Cal-Het:PV-Cre or Cal-Hom: PV-Cre (left). Active PV-positive neurons were labeled and confirmed by PV antibody staining. Scale bars, 50 μm. j Cell-type specific, light- and activity-dependent gene expressions were confirmed. Source data are provided as a Source Data file.