Fig. 1. Sirt7 KO mice display elevated energy expenditure and body temperature under normal conditions.

a, b Body weight (a) and percent epiWAT and iBAT weight calculated relative to body weight (b) in 15-week-old male WT and Sirt7 KO mice. p = 0.0286 (epiWAT), p = 0.0288 (iBAT). c–j Data of indirect calorimetry experiments from 15-week-old male WT and Sirt7 KO mice. VO₂ rates (c), average VO₂ (d), average VCO₂ (e), energy expenditure (f), RER (g), locomotor activity (h), water intake (i), and food intake (j). p = 0.0021 in (c); p = 0.0023 (Dark), p = 0.0082 (Light), p = 0.0021 (Total) in (d); p = 0.0255 (Dark), p = 0.0101 (Light), p = 0.0106 (Total) in (e); p = 0.0063 (Dark), p = 0.0049 (Light), p = 0.0026 (Total) in (f); p = 0.0296 in (j). k Representative H&E-stained sections of iBAT from 15-week-old male WT and Sirt7 KO mice (left panel, scale bar = 50 μm), and quantification of the lipid area (n = 3 independent animals per group) (right panel). p = 0.0347. l Oscillation of body temperature in 10-week-old male WT and Sirt7 KO mice. p = 0.0013. Data are presented as means ± SEM. All numbers (n) are biologically independent samples. Two-way ANOVA with Bonferroni’s multiple comparisons test (c, l); two-tailed Student’s t-test (a, b, d–k). *p < 0.05; **p < 0.01. Source data are provided as a Source Data file.