Fig. 4. SIRT7 deficiency in adipose tissue elevates whole-body energy expenditure and body temperature.

a, b Data of indirect calorimetry experiments and body temperature from 12-week-old male Adipoq-Cre control and Sirt7 AdKO mice. VO₂ rates (a) and energy expenditure (b). p = 3.7E–29 in (a); p = 0.0011 (Dark), p = 0.0030 (Light), p = 0.0006 (Total) in (b). c Oscillation of body temperature in 20-week-old male Adipoq-Cre control and Sirt7 AdKO mice. p = 0.0025. d Real-time qPCR analysis of BAT-related genes in iBAT of 12-week-old male Adipoq-Cre control and Sirt7 AdKO mice. p = 0.0089 (Pparg), p = 0.0239 (Ppargc1a), p = 0.0026 (Ebf2), p = 0.0005 (Dio2), p = 0.0010 (Cox3), p = 0.0006 (Cox8b), p = 5.0E–06 (Clstn3b), p = 0.0001 (S100b), p = 0.0002 (Bmp8b), p = 0.0003 (Fgf21), p = 0.0008 (Nrg4). e T3 level in iBAT of 11-week-old male Adipoq-Cre control and Sirt7 AdKO mice. p = 0.0420. f Western blot analysis of UCP1 in iBAT of 12-week-old male Adipoq-Cre control and Sirt7 AdKO mice (left panel). Quantification of the UCP1 protein bands relative to GAPDH control is shown on the right side. p = 0.0019. Data are presented as means ± SEM. All numbers (n) are biologically independent samples. Two-way ANOVA with Bonferroni’s multiple comparisons test (a, c); two-tailed Student’s t-test (b, d–f). *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source Data file.