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. 2022 Nov 29;13:1072271. doi: 10.3389/fendo.2022.1072271

Table 3.

Sex steroid priming regimens for boys.

Dose Route of administration Timing prior to GHST
Testosterone depot
(Testosterone Enanthate)
25 mg (47)
50 – 100 mg (58, 68)
100 mg (4, 55)
Intramuscular 7-10 days
7 days
5-8 days (55), 7-10 days (4)
Sustanon®
(mixture of Testosterone esters: propionate, phenylpropionate, isocaproate, decanoate)
50-100 mg (69)
62.5 mg/m2 (57, 67)
125 mg/m2 (57, 67)
MDT (multiple dose testosterone) 62.5 mg/m2 monthly for 3 doses (67)
Intramuscular 3-5 days
7 days
7 days
Last injection 7 days before test
Testosterone undecanoate 120 mg daily (13) Oral 5 days
Testosterone gel 2% 10 mg daily for 6 months (59, 60) Transdermal 3-6 months before test
β-oestradiol (oestradiol valerate) 1 mg < 20 kg
2 mg >20 kg daily (bedtime) (52, 56)
Oral For 2 (68) – 3 (52) days
Ethinyloestradiol 10 or 20 or 50 or 100 mcg (69)
20 mcg/m2 daily (bedtime) (61)
40 mcg/m2 daily (8)
50 mcg patch (62)
Oral
Oral
Oral
Transdermal
For 3 days
For 1-2 days
For 2 days
1 patch to be applied and kept on for 3 days
Stilboestrol 5 mg twice a day (63)
1 mg twice a day (64)
Oral
Oral
For 3 days
For 2 days
Premarin® (Conjugated oestrogen) 1.25 daily (61, 65)
2.5 mg (66)
Oral
Oral
For 3 days
1 dose the evening before and 1 dose the morning of the GHST