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. 2022 Nov 29;13:1074921. doi: 10.3389/fendo.2022.1074921

Figure 1.

Figure 1

Expression profile of Cyp17a2 in tilapia. (A), The specificity of polyclonal antibody against Cyp17a2 (~60 KD) in the testis, ovary, head-kidney, brain, and muscle was verified by Western blotting. T, testis; O, ovary; HK, head-kidney; B, brain; M, muscle. (B), Cellular localization of Cyp17a2 in the testes was detected by IHC. Cyp17a2 was expressed in the Leydig cells of the testis at 90, 180, and 210 dah. SG, spermatogonia; SC, spermatocytes; Lc, Leydig cells. (C), Cyp17a2 expression in the head-kidneys was detected by IHC. Cyp17a2 positive signals were detected in the interrenal cells of the head-kidney at 5, 10, 60, and 180 dah. Arrowhead, positive signals of Cyp17a2.