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. 2022 Nov 29;13:1074921. doi: 10.3389/fendo.2022.1074921

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Copyright © 2022 Yang, Wu, Su, Zhang, Chakraborty, Wang and Zhou

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of cyp17a2 deficiency on steroid production in testis of tilapia. (A), Immunohistochemistry of Cyp17a1 (a-d) and Cyp11b2 (a’-d’) in the testis of cyp17a2 ^+/+ and cyp17a2 ^-/- XY tilapia at 90 and 180 dah. (B), Expression level of cyp11b2 and cyp17a1 in the testis of cyp17a2 ^+/+ (n=3) and cyp17a2 ^-/- (n=3) fish at 90 (a) and 180 dah (b) detected by qRT-PCR. β-actin was used as a reference gene to normalize the expression values. (C), Expression of Cyp11b2 and Cyp17a1 analyzed by Western blotting (a) and quantification of protein level (b) in the testis of cyp17a2 ^+/+ and cyp17a2 ^-/- at 180 dah. (D, E), Serum 11-KT and DHP level in cyp17a2 ^+/+ (n=5) and cyp17a2 ^-/- (n=5) fish measured using an EIA kit. The data are reported as the means ± SD. Asterisk above the error bar indicate significant differences between groups tested by Student’s t-test (*, P<0.05).