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. 2022 Nov 29;10:1074990. doi: 10.3389/fbioe.2022.1074990

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Copyright © 2022 Bando, Yamashita, Tsumori, Minoura, Okumura and Hattori.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Automated long-term maintenance of iPSC. (A) All immunocytometorical analyses of the three independent experiments for Oct-3/4 and SSEA-4. Each blue histogram indicates no primary antibody control. Each red histogram represents the indicated antigen-specific signal. (B) iPSC expansion calculated using every automated cell count in the three independent experiments. (C) Representative images of immunohistochemically stained cells for Oct-3/4, SSEA-4, and Tra 1–61. Scale bars = 50 μm. (D) Karyotype of the RIKEN2F-iPSC after the fifth passage.