FIG. 1.
Bafilomycin A1 inhibition of toxin B-mediated cytopathic effects. CHO cells were incubated with toxin B (TB; 50 ng) in a 96-well plate, and bafilomycin A1 (I; 5 × 10−7 M) was added at 10-min intervals from 0 to 80 min. Each sample was performed in triplicate, and cytopathic effects were determined at 8 and 16 h. The error bars mark the standard deviation from the mean. Similar levels of inhibition were found in two subsequent repetitions of this experiment. B, phosphate-buffered saline control.