FIGURE 5.

Protection by PE-TS treatment in newborn mice from lethal EV71 challenge. (A) Inhibitory effect of filtered-PE-TS against EV71 infection in RD cells. The RD cells were infected with EV71 (MOI = 0.05) and exposed to filtered-PE-TS at the indicated concentrations (n = 3). The inhibitory effect was measured by cell viability assay at 48 hpi. The experiments were repeated twice independently. BALB/c lactating mice (n = 9–11 per group) were intracranially challenged with EV71 at a dose of 1.5 × 10⁶ TCID₅₀ within 24 h after birth and then administered PE-TS (unfiltered or filtered) via gavage or intraperitoneal injection at the indicated dose, respectively. Ribavirin (0.03 mg/g) was used as the medicine control group, and 10% DMSO was added with PBS as the non-infection control group. The mice were administered PE-TS once a day for seven consecutive days, and the survival rate (B), body weight (C), and clinical symptom scores (D) were recorded for 16 consecutive days post-EV71 challenge. Significance: *p < 0.1, **p < 0.05, ***p < 0.001 compared with virus control group.