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. 2022 Dec 12;5:1362. doi: 10.1038/s42003-022-04314-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Western blot analysis of L1CAM expression in ovarian cancer cell lines (a) primary ascites-derived HGSC cells (b) and immortalized fallopian tube cell lines (c) and a panel comparing immortalized fallopian tube cell lines to ovarian cancer cell lines (d). Actin was used as a loading control. Relative L1CAM expression was calculated by the ratio of the densitometry of L1CAM divided by Actin. e Immunofluorescence images of selected cell lines for surface L1CAM (green) and DAPI (blue) to stain the nucleus. f Relative migration in OVCAR8, CAOV3 and FT282-CE after siRNA knock-down of L1CAM or treatment with control siRNA. g Relative migration in FT33-RAS, FT189, FT237 and FT246, measured after overexpression of L1CAM or an empty control vector. e Scalebar represents 100 µm. f, g Three independent experiments were performed and P-values were calculated with an unpaired two-sided t-test. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001.