FIG. 2.

Physical characteristics of GTase-R. (A) SDS-PAGE of GTase preparations at different stages of purification. Lanes: 1, culture supernatant; 2, ammonium sulfate precipitate; 3, pooled active fractions from Q-ion-exchange chromatography; 4, pooled active fraction from CHT-10 hydroxylapatite chromatography; M, molecular mass markers. (B) Isoelectric focusing-PAGE of GTase-R. Lane: 1, pI markers (3.50 to 8.65); 2, Purified GTase-R visualized by PAS staining. (C) Effect of pH on GTase activity.