FIG. 3.

Effects of GTase-R on the cellular adhesion of S. mutans. S. mutans cells grown in BHI broth were resuspended at 0°C in 0.1 M KPB (pH 6.0) with 0.05% NaN₃ containing 1% sucrose. The cell suspension was mixed with increasing amounts of GTase-R and incubated at 37°C for 18 h at a 30° angle (resting cells). As a positive control, S. mutans was grown in BHI broth with 1% sucrose at 37°C for 18 h at a 30° angle (growing cells). Numbers of adhesive cells were then determined. Data are expressed as means and standard deviations of triplicate experiments. The asterisks indicate statistical significance (P < 0.01) from the sucrose value for resting cells incubated in sucrose containing KPB without GTase-R.