TABLE 2.
Effects of anti-S. oralis ATCC 10557 GTase antibody on the GTase activities of various oral streptococci
| GTase origina | GTase activity (dpm)b
|
% Inhibition | |
|---|---|---|---|
| Without anti-GTase-R | With anti-GTase-R | ||
| S. oralis | |||
| ATCC 10557 | 4,930.3 ± 12.8 | 831.8 ± 14.2 | 83.1 |
| SK23 | 4,997.9 ± 49.7 | 1,065.1 ± 42.2 | 78.7 |
| ATCC 9811 | 5,022.0 ± 37.6 | 1,128.8 ± 36.7 | 77.5 |
| S. sanguis | |||
| ATCC 10556 | 5,000.0 ± 100.0 | 1,305.4 ± 59.5 | 73.9 |
| ST3 | 5,027.1 ± 25.8 | 1,244.4 ± 36.1 | 75.3 |
| ST7 | 5,053.5 ± 21.8 | 1,243.8 ± 29.6 | 75.4 |
| S. gordonii | |||
| ATCC 10558 | 4,975.8 ± 37.9 | 949.4 ± 23.3 | 80.9 |
| 90A | 4,950.2 ± 32.9 | 1,468.6 ± 30.9 | 70.3 |
| SK51 | 4,956.5 ± 40.9 | 1,007.3 ± 58.9 | 79.7 |
| S. mutans | |||
| MT8148 cell-associated | 4,996.4 ± 53.3 | 4,687.9 ± 63.8 | 6.2 |
| MT8148 cell-free | 4,964.7 ± 72.5 | 3,431.3 ± 61.6 | 30.9 |
| S. sobrinus | |||
| 6715 | 5,006.9 ± 138.4 | 3,732.1 ± 194.9 | 25.5 |
| S. sobrinus | |||
| HHT | 4,989.9 ± 66.9 | 3,662.2 ± 76.5 | 26.6 |
a
GTase was from the concentrates of test strain culture supernatants, except for the cell-associated GTase from S. mutans. The cell-associated GTase was extracted from the cells of S. mutans with 8 M urea.
b
The GTase fraction (1 mU) was reacted with the antibody to GTase-R (32 μg of protein) at 37°C for 30 min or left unreacted. The reaction mixture was then incubated with [glucose-14C]sucrose at 37°C for 1 h, and the amount of synthesized [14C]glucan was measured. Data are expressed as means ± standard deviations of triplicate experiments.