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. 2022 Nov 29;13:1010796. doi: 10.3389/fendo.2022.1010796

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Copyright © 2022 Cannarella, Mancuso, Arato, Lilli, Bellucci, Gargaro, Curto, Aglietti, La Vignera, Condorelli, Luca and Calogero

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IGF2 mRNA and protein are expressed in human spermatozoa. RT-PCR analysis (A), immunoblots (B), and densitometric analysis (C) of IGF2 in human spermatozoa. MA5-17096, clone 8H1, detects IGF2 which has a predicted molecular weight of approximately 20.1 kDa. The observed molecular weight of the protein may vary from the mentioned predicted molecular weight due to post-translational modifications, post translation cleavages, relative charges, and other experimental factors, as shown by the presence of other bands at the highest molecular weight (in particular at 35 KDa). Data represent the mean ± SD of three independent experiments, each performed in triplicate. Porcine Sertoli cells (SCs) were used as a positive control. Magnification 100x.