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. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: Hepatology. 2022 Jul 5;77(1):159–175. doi: 10.1002/hep.32604

FIGURE 1.

FIGURE 1

Decreased dynamin-related protein 1 (DRP1) expression in human alcoholic hepatitis (AH) and mouse alcohol-associated liver disease liver. (A) Total liver lysates and RNA from patients with AH and healthy donors were subjected to western blot analysis. (B) Densitometry analysis of (A). (C) Real-time quantitative PCR (qPCR) analysis. (D) Representative electron microscopy images of the livers from healthy donors and patients with AH. Arrowheads denote megamitochondria. LD, lipid; M, mitochondria; N, nuclear. (E) Mitochondrial length and width were measured from (D) using Image-Pro Plus software (mitochondria number = 700–800 from n = 3 livers). (F) Male wild-type C57BL/6J mice (2–3 months old) were subjected to Gao-binge alcohol feeding. Total liver lysates and RNA were subjected to western blot analysis, (G) Densitometry analysis of (F) and (H) qPCR analysis. All data are presented as means ± SEM (n = 4–9). *p < 0.05, **p < 0.01; two-tailed Student’s t test. EtOH, ethanol; MFN, mitofusin; mRNA, messenger RNA.