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. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: Hepatology. 2022 Jul 5;77(1):159–175. doi: 10.1002/hep.32604

FIGURE 3.

FIGURE 3

Gao-binge alcohol induces megamitochondria via downregulation of dynamin-related protein 1 (DRP1) in mouse liver. Male 2–3-month-old liver-specific DRP1 knockout and matched littermates were subjected to Gao-binge alcohol feeding. Total liver lysates were subjected to western blot analysis (A). (B) Densitometry analysis of (A). Data are presented as means ± SEM (n = 3–4). (C) Representative mouse liver electron microscopy (EM) images. Arrows denote megamitochondria. LD, lipid; M, mitochondria; N, nuclear. (D) Mitochondrial length and width from (C) were measured using Image-Pro Plus software (n = 1000–2000 from 2–3 mice). (E) Number of mitochondria per EM field were quantified and normalized with the cytosol area. At least 10 fields from each mouse were quantified. Data are presented as means ± SD. MFN, mitofusin. *p < 0.05, **p < 0.01, one-way analysis of variance analysis with Bonferroni’s post hoc test.