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. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: Hepatology. 2022 Jul 5;77(1):159–175. doi: 10.1002/hep.32604

FIGURE 4.

FIGURE 4

Loss of hepatic dynamin-related protein 1 (Drp1) exacerbates Gao-binge alcohol-induced liver injury but not steatosis in mice. Male 2–3-month-old liver-specific DRP1 knockout and matched littermates were subjected to Gao-binge alcohol feeding. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels (A, B) and (C) liver triglyceride (TG) levels were measured. Data are presented as means ± SEM (n = 4–7). (D) Representative liver hematoxylin and eosin, (E) Oil Red, and Sirius Red (F) staining images are shown. (G) Quantification of Sirius Red staining positive areas using Image J. Data are presented as means ± SD (n = 15 different fields of 3 mice). (H) Liver hydroxyproline content was measured. (I) Real-time quantitative PCR analysis of the fibrogenic genes in mouse livers. Data are presented as means ± SEM (n = 4–5). *p < 0.05; **p < 0.01; ***p < 0.001; one-way analysis of variance analysis with Bonferroni’s post hoc test.