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. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: Hepatology. 2022 Jul 5;77(1):159–175. doi: 10.1002/hep.32604

FIGURE 6.

FIGURE 6

Liver-specific DRP1 knockout (L-DRP1 KO) mice have impaired mitochondrial adaptation to EtOH. Male 2–3-month-old L-DRP1 KO and matched littermates were subjected to Gao-binge alcohol feeding. (A) Pooled mouse liver mitochondria were subjected to bioenergetics analysis using the Seahorse Bioscience XF analyzer (n = 2–4). (B) Quantification of state 3 respiration from (A). (C) Liver cytosol fractions were subjected to western blot analysis. (D) Principal component analysis (PCA) of metabolomic data from indicated mouse liver. (E) Heatmap of fatty acid metabolism of metabolomics analysis of indicated mouse liver tissues (n = 6). (F) PCA of RNA-sequencing (RNA-seq) data. The RNA-seq data are accessible with GSE207074. (G) Heatmap analysis of fatty acid beta-oxidation genes from the RNA-seq dataset. ADP, adenosine diphosphate; EtOH, ethanol; FCCP, carbonyl cyanide p-trifluoromethoxyphenylhydrazone; OCR, oxygen consumption rate; PC, principal component.