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. 2022 Nov 29;9:974938. doi: 10.3389/fnut.2022.974938

TABLE 2.

Hazard ratio (HR) (95% confidence intervals [CIs]) for all-cause and cause-specific mortality according to serum methylmalonic acid (MMA) concentrations among patients with diabetes in National Health and Nutrition Examination Survey (NHANES).

Serum MMA concentrations, nmol/L
Causes of death <120 120–175 175–250 ≥250 p-trend Per one-unit increment innatural log-transformed MMA
All causes mortality
Number of deaths/total 131/927 252/1,012 188/572 272/586
Crude 1.000 (ref.) 1.927 (1.559–2.380) 3.234 (2.586–4.045) 5.609 (4.546–6.921) 0.000 4.596 (3.891–5.428)
Model 1 1.000 (ref.) 1.466 (1.183–1.816) 2.005 (1.590–2.527) 3.097 (2.477–3.873) 0.000 2.977 (2.440–3.633)
Model 2 1.000 (ref.) 1.287 (0.888–1.865) 1.665 (1.082–2.561) 2.119 (1.395–3.219) 0.003 2.443 (1.472–4.053)
Model 3 1.000 (ref.) 1.225 (0.839–1.789) 1.713 (1.104–2.657) 2.181 (1.421–3.347) 0.002 2.683 (1.575–4.572)
CVD mortality
Number of deaths 33 73 54 82
Crude 1.000 (ref.) 1.933 (1.387–2.694 2.932 (2.050–4.194) 5.116 (3.663–7.145) 0.000 4.383 (3.335–5.760)
Model 1 1.000 (ref.) 1.490 (1.065–2.086) 1.851 (1.279–2.680) 2.914 (2.042–4.157) 0.000 2.902 (2.093–4.025)
Model 2 1.000 (ref.) 2.470 (1.159–5.264) 1.008 (0.359–2.827) 2.736 (1.156–6.476) 0.016 3.361 (1.663–6.796)
Model 3 1.000 (ref.) 2.268 (1.053–4.883) 0.991 (0.348–2.825) 2.509 (1.044–6.027) 0.038 2.062 (1.728–5.842)
Cancer mortality
Number of deaths 26 45 26 34
Crude 1.000 (ref.) 1.712 (1.056–2.776) 2.167 (1.256–3.738) 3.310 (1.978–5.538) 0.000 3.322 (2.037–5.419)
Model 1 1.000 (ref.) 1.394 (0.853–2.277) 1.498 (0.852–2.634) 2.057 (1.190–3.553) 0.008 2.190 (1.226–3.910)
Model 2 1.000 (ref.) 1.418 (0.564–3.564) 1.968 (0.713–5.429) 3.973 (1.532–10.301) 0.017 4.755 (1.694–13.346)
Model 3 1.000(ref.) 1.230 (0.472–3.205) 2.000 (0.717–5.578) 4.242 (1.537–11.712) 0.015 4.515 (1.462–13.947)

Model 1: adjusted for age (continuous), sex (male, or female), and race/ethnicity (non-Hispanic white, non-Hispanic black, Mexican American, or other).

Model 2: further adjusted (from Model 1) for BMI (<25.0, 25.0–30, or ≥30 kg/m2), education level (less than high school, high school or equivalent, or college or above), family income-poverty ratio (≤1.0, 1.0–3.0, or >3.0), Alcohol drinks (continuous), smoking status (never smoker, ever smoker, or current smoker), and Physical activity (vigorous activity, moderate activity, or Inactive).

Model 3: further adjusted (from Model 2) for Vitamin B12 (pmol/L, continuous), diabetes medication use (no insulin or pills, only diabetes pills, only insulin, or pills and insulin), Measurement of serum MMA (gas chromatography-mass spectrometry, standardized liquid chromatography-tandem mass spectrometry).