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. 2022 Nov 29;13:1068438. doi: 10.3389/fpls.2022.1068438

Figure 4.

Figure 4

ALD1, NPR1, and SID2 are necessary to fully express RPS2-mediated immunity against an avirulent Pseudomonas infection. (A) Free SA levels in the leaves of WT, ald1, npr1, ald1npr1, sid2, and ald1sid2 plants during avirulent PsmES4326/AvrRpt2 infection. Data show averages with standard deviations from five biological replications (n = 5). (B) Deregulation of PR1 transcription in ald1npr1 and ald1sid2 mutants after infection. PR1 expression levels relative to an ACTIN2 gene in non-inoculated leaves of WT plant was set to 1. The columns and bars show the average with standard deviation obtained from three biological replications (number of technical replicates = 3). (C) Relative level of FRK1 mRNA in leaves of Arabidopsis plants infected with PsmES4326/AvrRpt2. We normalized expression levels and presented the results mentioned above (B). (D) Level of BAK1 in leaves of WT and mutants in the absence or presence of avirulent pathogen infection. Upper, BAK1 protein levels in leaves of non-infected Arabidopsis plants; lower, BAK1 levels after PsmES4326/AvrRpt2 infection. Bands not indicated are non-specific. Graphs on the right side of D show the normalized BAK1 levels in Arabidopsis at the indicated time points (average ± standard deviation, n = 3, or 4). Different letters indicate statistically significant differences (p < 0.05 [D], p < 0.01 [A-C], one-way ANOVA).