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. 2022 Nov 29;12:1061790. doi: 10.3389/fcimb.2022.1061790

Figure 1.

Figure 1

LPS-stimulated inflammatory reaction and MiR-199a-3p expression in vivo and in vitro. RAW264.7 cells were stimulated with or without LPS for 12, 24, 48, and 72 h. (A) The effects of various concentrations of LPS on the viability of RAW264.7 cells after 24 h treatment, as tested via CCK-8 assay (n = 3). (B) The effect of LPS (100 ng/ml) on the level of IL-6 in cell supernatants, as tested via ELISA (n = 3). (C) The effect of LPS (100 ng/ml) on the level of TNF-α in cell supernatants, as tested via ELISA (n = 3). (D) qRT-PCR detection of MiR-199a-3p expression in RAW264.7 cells after LPS treatment (n = 3). (E) qRT-PCR detection of MiR-199a-3p expression in alveolar macrophages after LPS treatment (n = 3). (F) MiR-199a-3p expression in BALF of LPS-induced ARDS or control mice was detected by qRT-PCR (n = 6). The experiments were repeated at least three times. Each value represents the mean ± SD of three independent experiments. *p < 0.05 vs. control group, Student’s t-test, or one-way ANOVA.