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. 2022 Nov 29;12:1061790. doi: 10.3389/fcimb.2022.1061790

Figure 5.

Figure 5

MiR-199a-3p mimics augmented LPS-induced SAP secretion via the activation of Rab8a in vitro. (A) Western blot analysis demonstrated the Rab8a levels in RAW264.7 cells with the transfection of MiR-199a-3p mimics or inhibitors. (B) Western blot analysis illustrated the efficient siRNA-mediated knockdown of Rab8a in RAW264.7 cells with the transfection of MiR-199a-3p mimics or inhibitors. (C) NTA revealed significant decreases in vesicle levels from RAW264.7 cells with Rab8a knockdown in the MiR-199a-3p mimic and inhibitor groups compared with the control group. (D) Western blot analysis demonstrated significant decreases in LC3-II levels in vesicles from LPS-stimulated RAW264.7 cells in the Rab8a knockdown groups compared with the control group. The experiments were repeated at least three times (n = 3 per group). Each value represents the mean ± SD of three independent experiments. *p < 0.05 vs. the control; #p < 0.05 vs. the LPS control; **p < 0.05 vs. the mimics group without Rab8a knockdown; ***p < 0.05 vs. the inhibitors group without Rab8a knockdown, one-way ANOVA.