TABLE 3.
Diagnostic performance evaluation of candidate biomarkers.
| Biomarker | Scan mode | Rt (s) | m/z | Adducts | AUC (95%CI) | Se (%) | Sp (%) | YI |
|---|---|---|---|---|---|---|---|---|
| RCC vs. BKT | ||||||||
| 3-β-D-Galactosyl-sn-glycerol | — | — | — | — | 0.989 (0.936–1.000) | 93.33 | 100.00 | 0.933 |
| γ-Aminobutyryl-lysine | — | — | — | — | 0.981 (0.924–0.999) | 93.33 | 92.11 | 0.854 |
| 7,8-Dihydroneopterin | — | — | — | — | 0.941 (0.867–0.981) | 93.33 | 84.21 | 0.775 |
| LPC 19:2 | — | — | — | — | 0.845 (0.748–0.916) | 77.27 | 78.38 | 0.557 |
| RCC vs. HC | ||||||||
| 3-β-D-Galactosyl-sn-glycerol | ESI- | 481.035 | 253.083 | M-H | 0.990 (0.942–1.000) | 97.73 | 100.00 | 0.977 |
| γ-Aminobutyryl-lysine | ESI- | 545.151 | 230.155 | M-H | 0.962 (0.898–0.991) | 86.36 | 95.56 | 0.819 |
| 7,8-Dihydroneopterin | ESI- | 481.173 | 254.086 | M-H | 0.916 (0.838–0.964) | 97.73 | 73.33 | 0.711 |
| LPC 19:2 | ESI+ | 440.903 | 556.346 | M + Na | 0.909 (0.829–0.959) | 93.18 | 80.00 | 0.732 |
| 6-Keto-prostaglandin F1α | ESI+ | 668.909 | 371.242 | M + H | 0.897 (0.815–0.952) | 88.64 | 82.22 | 0.709 |
| 17α,21-Dihydroxypregnenolone | ESI+ | 698.951 | 349.237 | M + H | 0.830 (0.735–0.901) | 77.27 | 77.78 | 0.551 |
| γ-Glutamylphenylalanine | ESI- | 283.958 | 293.113 | M-H | 0.823 (0.728–0.896) | 77.27 | 80.00 | 0.573 |
| RCC vs (BKT + HC) | ||||||||
| 3-β-D-Galactosyl-sn-glycerol | — | — | — | — | 0.990 (0.953–0.999) | 97.78 | 97.56 | 0.953 |
| γ-Aminobutyryl-lysine | — | — | — | — | 0.971 (0.924–0.992) | 86.67 | 95.12 | 0.818 |
| 7,8-Dihydroneopterin | — | — | — | — | 0.928 (0.868–0.966) | 95.56 | 78.05 | 0.736 |
| LPC 19:2 | — | — | — | — | 0.879 (0.808–0.930) | 93.18 | 71.60 | 0.648 |
| (RCC + BKT) vs HC | ||||||||
| 3-β-D-Galactosyl-sn-glycerol | — | — | — | — | 0.763 (0.679–0.834) | 65.06 | 93.18 | 0.582 |
| γ-Aminobutyryl-lysine | — | — | — | — | 0.692 (0.604–0.771) | 48.19 | 97.73 | 0.459 |
| 7,8-Dihydroneopterin | — | — | — | — | 0.691 (0.602–0.770) | 63.86 | 72.73 | 0.366 |
| LPC 19:2 | — | — | — | — | 0.803 (0.722–0.869) | 81.48 | 70.45 | 0.519 |
Note: HC, healthy control; BKT, benign kidney tumor; RCC, renal cell carcinoma; Rt, retention time; m/z, mass-to-charge ratio; LPC, lysophosphaticholine; AUC, area under ROC, curve; 95%CI, 95%confidence interval; Se, sensitivity; Sp, specificity; YI, youden index.