TABLE 2.
Phenotype of selected P. aeruginosa 4020::Tn5G phagocytosis-deficient mutants
| Mutanta | Characteristics
|
||
|---|---|---|---|
| Ingestionb | Motilityc | Phage PO4d | |
| H27A* (H24F) | Low | + | s |
| T3B* (T5C) | Low | + | s |
| T25B* (H7F, H8H) | Low | + | s |
| T15C* (T29F, T41F) | Low | + | s |
| T36B | Low-moderate | + | s |
| T7C | Low-moderate | + | s |
| T41B | Low-moderate | + | s |
| H39F* (H18F, H33F) | Low-moderate | + | r |
| H5E | Low-moderate | + | r |
| T13H | Low-moderate | − | s |
| T32F | Low-moderate | + | s |
| T49C | Moderate | + | s |
| H49E | Moderate | + | s |
| T2E | Moderate | − | s |
| PAK-N1G (rpoN::Gm) | Low | − | r |
a
An asterisk indicates that the transposon plus flanking DNA has been cloned from this mutant. Mutants in parentheses are siblings of the respective mutants (Southern blots). Mutants with the H designation were isolated from a shaking pool; mutants with the T designation were isolated from a static pool.
b
The initial screen of ingestion was graded: low, 25% of the parental P. aeruginosa 4020 strain; low-moderate, 25 to 40% of the parental strain; and moderate, ∼50% of the parental strain.
c
Motility was determined on L broth–0.3% agar plates.
d
s, sensitive to PO4 infection; r, resistant to PO4.