Skip to main content
. 2022 Nov 29;9:961354. doi: 10.3389/fvets.2022.961354

Figure 5.

Figure 5

The 50K genomic island integration and excision. (A) The primer pairs P3/P4, P5/P6 were used for detecting genomic island excision, and P3/P5, P4/P6 were used for detecting genomic island integration in R. anatipestifer ATCC 11845, RA-YM, and clinical isolated strain no. 1. M: DL2000 DNA marker; lane 1: DNA fragment was amplified with P3/P4 primer pair from R. anatipestifer ATCC11845; lane 2: DNA fragment was amplified with P5/P6 primer pair from R. anatipestifer ATCC11845; lane 3: DNA fragment was amplified with P4/P6 primer pair from R. anatipestifer ATCC11845; lane 4: DNA fragment was amplified with P3/P5 primer pair from R. anatipestifer ATCC11845; lanes 5–8: DNA fragment was amplified with same primer from R. anatipestifer YM; lanes 9–12: DNA fragment was amplified with same primer from R. anatipestifer clinical isolated strain no. 1; lane 13: negative control; R. anatipestifer ATCC 11845 and RA-YM genomic islands had the integration and excision function. (B) Schematic representation of the integration and excision model.