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Expression of TetC-Sh28GST fusion and Sh28GST as determined by SDS-PAGE and Western blotting. Shown are the results of probing with a rabbit anti-TetC polyclonal antiserum (A) and a mouse anti-Sh28GST polyclonal antiserum (B). The constructs were in S. enterica serovar Typhimurium strains SL5338 and SL3261, as indicated. Lane M, low-molecular-mass marker proteins (kilodaltons). Lanes 1 to 8, SL5338, JJ502, JJ503, JJ504, SL3261, JJ702, JJ703, and JJ704 cell lysate, respectively.
