IFN-γ production by whole spleen cells after stimulation with rTetC (A) and rSh28GST (B). Results are expressed as the concentration of cytokine in supernatants of cell cultures collected up to 8 days after stimulation, as detected by ELISA. Each value is the average of the results for three different cultures plus the standard deviation. Statistical significances were analyzed by Student's t test. P values <0.05 were considered significant. Groups: I, naive mice; II, mice immunized with SL3261; III, mice immunized with JJ702; IV, mice immunized with JJ703; V, mice immunized with JJ704.