TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Characteristics | Source or reference |
|---|---|---|
| Strains | ||
| E. coli TG2 | recA | 33 |
| S. enterica serovar Typhimurium | ||
| SL5338 | galE r− m+ | 7 |
| JJ502 | SL5338 harboring pTECH2 plasmid | This study |
| JJ503 | SL5338 harboring pTECH2-Sh28 plasmid | This study |
| JJ504 | SL5338 harboring pTECH10-Sh28 plasmid | This study |
| SL3261 | SL1344 aroA | 20 |
| JJ702 | SL3261 harboring pTECH2 plasmid | This study |
| JJ703 | SL3261 harboring pTECH2-Sh28 plasmid | This study |
| JJ704 | SL3261 harboring pTECH10-Sh28 plasmid | This study |
| Plasmids | ||
| pTECH2 | pnirB TetC fusion vector | 25 |
| pTECH2-Sh28 | pTECH2 carrying Sh28GST ORFa | This study |
| pTECH10-Sh28 | pnirB Sh28GST expression plasmid | This study |
a
ORF, open reading frame.