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. 2022 Dec 13;13:7703. doi: 10.1038/s41467-022-35492-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Representative images of lungs and H&E-stained tissue sections at 24 h after LPS stimulation. Scale bars in the upper panel, 1 mm; scale bars in the below panel, 50 µm. b Lung damage was determined by 5 pathophysiological features to obtain the total injury score, n = 6 in each group. c The permeability of alveolocapillary membrane was evaluated by the lung wet/dry (W/D) ratio, n = 6 in each group. d–i Immunohistochemical staining and quantitative analysis of Ly6G (d, e, n = 7 in each group), MPO (f–g, n = 7 in each group) and NE (h, i, n = 6 in each group) in mouse lung samples. ns, not significant. All samples were biologically independent and three or more independent experiments with similar results were performed. Data are presented as mean ± SEM and analyzed with a 95% confidence interval. Statistical analysis was performed using two-way ANOVA followed by Bonferroni’s post hoc test. Source data are provided as a Source Data file.