Fig. 7. IL-10 depletion leads to poor prognosis of ALI through the persistent harmful lung Fth1^hi Neu phenotype.

a–f On day 4 and day 7 after LPS challenge, BALF and serum were collected from WT and Il-10^−/− mice for determining the levels of cytokines: IL-6 (a, n = 4, 4, 5, 5 for BALF and n = 6, 6, 5, 5 for serum), IFN-γ (b, n = 4, 4, 5, 5 for BALF and n = 5, 5, 4, 4 for serum), IL-12p70 (c, n = 5 in each group for BALF and serum), IL-1β (d, n = 4, 4, 5, 5 for BALF and n = 5, 5, 4, 4 for serum), G-CSF (e, n = 4 in each group for BALF and serum), and IL-17A (f, n = 4 in each group for BALF and serum). g, h Representative images of H&E-stained tissue sections at 4 d (g) and 7 d (h) after LPS stimulation. Scale bars in the upper panel, 1 mm; scale bars in the below panel, 50 µm. i–l Representative images of anti-Fth1/anti-Ly6G (i, j) and anti-Prok2/anti-Ly6G (k, l) immunofluorescence-stained lung tissues of WT and Il-10^−/− mice at 4 d (i, k) and 7 d (j, l) after LPS challenge. The nuclei were stained with DAPI, displayed in blue. Scale bars, 50 µm. All samples were biologically independent and three or more independent experiments with similar results were performed. Data are presented as mean ± SEM and analyzed with a 95% confidence interval. Statistical analysis was performed using two-tailed unpaired Student t test. Source data are provided as a Source Data file.