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. 2022 Oct 27;13(6):3014–3027. doi: 10.1002/jcsm.13093

Figure 3.

Figure 3

(A) Intestinal permeability (barrier dysfunction) as measured by FITC‐dextran, (B) endotoxin (LPS) concentration in the plasma and (C) plasma IL‐6 concentration (marker for systemic inflammation). Serum fluorescein dextran concentrations (λex = 485; λem = 535 nm) were measured 4 h after gavage. (D) qRT‐PCR analysis of biomarkers involved in intestinal permeability and integrity of tight junctions and mucous barrier (Cldn1, Muc2, Ocln and Tjp1) and (E) qRT‐PCR analysis of inflammatory cytokine and chemokine transcripts in the hypothalamus in KPC and 4662 PDAC‐bearing animals at 15–16 days after orthotopic implantation. mRNA levels are relative to sham group. N = 7–9, 7–8, 6–7, 4–6 in sham, KPC, 4662 and DSS groups, respectively. *P < 0.05, **P < 0.01, ***P < 0.001 compared with sham; $ P < 0.05, $$P < 0.01, $$$P < 0.001 compared with 4662. # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001 compared with DSS. One‐way ANOVA in (A, B and C) analysis of variance with post hoc Bonferroni's multiple comparison test. Two‐way ANOVA in (D and E) analysis of variance with post hoc Bonferroni's multiple comparison test.