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. 2022 Oct 27;13(6):3014–3027. doi: 10.1002/jcsm.13093

Figure 5.

Figure 5

C26 tumour secretome mimic results in inflammatory responses in hypothalamic cells. (A) IL‐6 secretion of mHypoE‐N46 cells incubated with C26 tumour secretome (C26) and variations on the C26 tumour secretome mimic, combined with LPS‐supplemented culture medium (+LPS), compared with the positive control (C26). (B) IL‐6 secretion of mHypoE‐N46 cells incubated with different concentrations PGE2 (6500–0 pg/mL), combined with LPS. The different concentrations were either provided to the cells in regular culture medium (PGE2 solely) or culture medium containing the other C26 mimic components (PGE2 within C26 mimics). IL‐6 (C), LIF (D) and CCL2 (E) secretion of mHypoE‐N46 cells incubated with C26 tumour secretome or a C26 mimic, in the presence or absence of LPS, compared with the positive control (LPS). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. One‐way ANOVA analysis of variance with post hoc Bonferroni's multiple comparison test.