Figure 7.

IL‐6 secretion of mHypoE‐N46 cells incubated with the tumour secretome of C26 cells (A), LLC cells (B), KPC cells(C) or 4662 cells (D), which were cultured with CXB. Hypothalamic cells were incubated with these medium combined with culture medium as well as LPS. As a control, cells were incubated with tumour secretome to which CXB was added after removal of the tumour cells combined with LPS (tumour med + CXB + LPS). Other control conditions included culture medium supplemented with LPS and CXB (LPS + CXB), culture medium supplemented with LPS (LPS) and regular culture medium (DMEM), compared with the positive control (LPS). *P < 0.05, **P < 0.01, ***P < 0.001. One‐way ANOVA analysis of variance with post hoc Bonferroni's multiple comparison test.